Journal: Channels

Article Title: Association of the chemerin-CMKLR1 with atrial potassium current dysregulation and atrial fibrillation in obese mice

doi: 10.1080/19336950.2025.2611704

Figure Lengend Snippet: Localization and expression of potassium channel-associated proteins in mouse atrial myocytes. A. Immunofluorescence images showing membrane localization and expression of Kv4.3, Kv4.2, KChIP2, Kv1.5, and Kir2.1 in atrial cardiomyocytes from LFD and HFD groups. Scale bar, 100 μm. B. Representative Western blotting images and densitometric quantification of Kv4.3, Kv4.2, KChIP2, Kv1.5, and Kir2.1 protein expression in atrial cardiomyocytes from LFD and HFD mice. LFD, low-fat diet; HFD, high-fat diet. Data are presented as mean ± SD ( n = 3). ns, not significant; * p < 0.05, ** p < 0.01 vs. LFD group.

Article Snippet: Primary antibodies diluted in blocking buffer were applied overnight at 4°C as follows: resistin (1:1,000; Abcam, USA), chemerin (1:1,000; Abcam, USA), leptin (1:1,000; Abcam, USA), CMKLR1 (1:1,000; InvitrogenTM, USA), Kv4.3 (1:1,000; Alomone Labs, Israel), Kv4.2 (1:1,000; Alomone Labs, Israel), KChIP2 (1:1,000; Alomone Labs, Israel), Kv1.5 (1:1,000; Alomone Labs, Israel), Kir2.1 (1:1,000; Alomone Labs, Israel), β-Tubulin (1:1,000; Abcam, USA).

Techniques: Expressing, Immunofluorescence, Membrane, Western Blot

Journal: Scientific Reports

Article Title: The Kv4 potassium channel modulator NS5806 attenuates cardiac hypertrophy in vivo and in vitro

doi: 10.1038/s41598-024-70962-x

Figure Lengend Snippet: Effects of NS5806 on I to channel-related gene and protein expression. ( A – C ) Quantification of the mRNA levels of Kv4.2 ( A ), KChIP2 ( B ) and DPP6 ( C ). GAPDH served as an internal control (n = 9 in each group). ( D ) Representative immunoblots. Original blots are presented in Supplementary Figure . ( E – G ) Summary data of Kv4.2 ( E ), KChIP2 ( F ) and DPP6 ( G ) protein levels. GAPDH served as an internal control (n = 5 in each group). * P < 0.05, ** P < 0.01 versus the Sham + Vehicle group. # P < 0.05, ## P < 0.01 versus TAC + Vehicle.

Article Snippet: Primary antibodies against Kv4.2 (1:500, 75–016, NeuroMab, USA), KChIP2 (1:500, DF14620, Affinity, China), DPP6 (1:200, APC-146, Alomone, Israel) and GAPDH (1:10,000, 10494-1-AP, Proteintech, China) were incubated at 4 °C overnight.

Techniques: Expressing, Control, Western Blot

Journal: European journal of pharmacology

Article Title: Dapagliflozin: A sodium-glucose cotransporter 2 inhibitor, attenuates angiotensin II-induced atrial fibrillation by regulating atrial electrical and structural remodeling.

doi: 10.1016/j.ejphar.2024.176712

Figure Lengend Snippet: Fig. 6. DAPA alleviated ion channel disorders and atrial fibrosis caused by Ang II. A. Immunoblotting analysis of the protein expression of CAMKII, ox-CAMKII, Nav1.5, Kv4.3, Kv4.2, Kchip2, Kir2.1, Cx40, smad2/3, p-smad2/3, TGF-β1 in atrial tissue. B, C. Quantification of CAMKII, ox-CAMKII expression level in atrial tissue. n = 4. *p < 0.05 vs. CTL group and #p < 0.05 vs. Ang II group. D-I. Quantification of atrial electrical activity associated protein expression level. n = 4. *p < 0.05 vs. CTL group and #p < 0.05 vs. Ang II group. J, K. Quantification of atrial fibrosis associated protein expression level. n = 4. *p < 0.05 vs. CTL group and #p < 0.05 vs. Ang II group. Statistical significance was assessed by one-way ANOVA analysis followed by Tukey tests or Kruskal-Wallis post hoc multicomparison test (B–K). Data are presented as means ± SEM.

Article Snippet: Protein lysates of tissue or cells were separated by 8% SDS-PAGE and transferred to NC membrane, and blocked for 2 h using 5% non-fat milk and probed with primary polyclonal antibodies against CaMKII (Abclonal), oxCaMKII (GeneTex), Nav1.5 (Abmart), Kv4.3 (Abclonal), Kv4.2 (Cell Signaling Technology), Kchip2 (Abclonal), Kir2.1(Zenbio), Cx40 (GeneTex), TGF-β1(Santa cruz), smad (Zenbio), p-smad (Zenbio) at 4 ◦C overnight.

Techniques: Western Blot, Expressing, Activity Assay

Journal: European journal of pharmacology

Article Title: Dapagliflozin: A sodium-glucose cotransporter 2 inhibitor, attenuates angiotensin II-induced atrial fibrillation by regulating atrial electrical and structural remodeling.

doi: 10.1016/j.ejphar.2024.176712

Figure Lengend Snippet: Fig. 7. SGLT2i alleviated ion channel disorders and atrial fibrosis caused by activation of CAMKII. A-I Immunoblotting analysis and quantitation of the protein expression of CAMKII, ox-CAMKII, Nav1.5, Kv4.3, Kv4.2, Kchip2, Kir2.1, Cx40 in HL-1 atrial myocytes. n = 4. *p < 0.05 vs. CTL group and #p < 0.05 vs. Ang II group. J-N. Immunoblotting analysis and quantitation of the protein expression of CAMKII, ox-CAMKII, smad2/3, p-smad2/3, TGF-β1 in primary mouse fibroblast. n = 4. *p < 0.05 vs. CTL group and #p < 0.05 vs. Ang II group. Statistical significance was assessed by one-way ANOVA analysis followed by Tukey tests or Kruskal- Wallis post hoc multicomparison test (B–I and K–N). Data are presented as means ± SEM.

Article Snippet: Protein lysates of tissue or cells were separated by 8% SDS-PAGE and transferred to NC membrane, and blocked for 2 h using 5% non-fat milk and probed with primary polyclonal antibodies against CaMKII (Abclonal), oxCaMKII (GeneTex), Nav1.5 (Abmart), Kv4.3 (Abclonal), Kv4.2 (Cell Signaling Technology), Kchip2 (Abclonal), Kir2.1(Zenbio), Cx40 (GeneTex), TGF-β1(Santa cruz), smad (Zenbio), p-smad (Zenbio) at 4 ◦C overnight.

Techniques: Activation Assay, Western Blot, Quantitation Assay, Expressing

Journal: Cells

Article Title: Influence of Age on Hyperoxia-Induced Cardiac Pathophysiology in Type 1 Diabetes Mellitus (T1DM) Mouse Model

doi: 10.3390/cells12111457

Figure Lengend Snippet: Protein profiles of key potassium channels and sex hormone receptors in hearts of aged Akita mice. Key potassium channels and their auxiliary proteins such as KChIP2, Kv1.4, Kv1.5, and Kv4.2 and housekeeping gene GAPDH ( a ), and androgen receptor (AR) and estrogen receptors (ER-α and β) ( b ). Values shown in the tables are mean (±SE) normalized band intensities from n = 4 per group. * represents p < 0.05 and ** p < 0.005 between normoxia and hyperoxia.

Article Snippet: Blots were later blocked in 5% w / v nonfat dry milk for 1 h and probed in 1:5000 dilutions of primary antibodies for Kv1.4 (AB5926) and GAPDH (Millipore) antibodies, and 1:1000 dilutions of KChIP2 (Abcam, Cambridge, MA, USA), Kv4.2 (Millipore, Billerica, MA, USA), estrogen receptor beta (Thermo Fisher, CA, USA), androgen receptor (Abcam, Cambridge, MA, USA), and Kv1.5 antibody (Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: